Mouse anti Human Bence Jones kappa (surface and hidden determinants), conjugated with FITC

Catalogue Number: MAHU/BJK/FITC-NMB

Manufacturer:Nordic MuBio
Type:Monoclonal Secondary Antibody - Conjugated
Shipping Condition:RT
Unit(s): 0.2 mg
Host name: Mouse
Clone: NI 285, NI 250
Isotype: IgG1
Immunogen:

Description

Description: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining and ELISA and immunoblotting using an indirect technique with monoclonal anti FITC labelled with peroxidase. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Additional Text

Antibody Clonality

Monoclonal

Application Notes

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Storage Note

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4C, not refrozen, and preferably used the same day.