Mouse anti Human IgA2 (A2(m)2 allotype specific)

Catalogue Number: MAHU/IGA2(M)2-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	0.5 mg
Host name:	Mouse
Clone:	NI 194-3 (A89-040)
Isotype:	IgG1
Immunogen:
Application:	ELISA, ICC, IHC, IB

Overview

Description

Description: The reactivity of the antiserum is restricted to the IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting.

Additional Text

Antibody Clonality

Monoclonal

Application Notes

Indirect immunofluorescence,ELISA. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:200 upwards; in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

Storage Note

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4C, not refrozen, and preferably used the same day.

Price

£417.00

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