Mouse anti Human IgA2 (A2(m)2 allotype specific), conjugated with FITC

Catalogue Number: MAHU/IGA2(M)2/FITC-NMB

Manufacturer:Nordic MuBio
Type:Monoclonal Secondary Antibody - Conjugated
Shipping Condition:RT
Unit(s): 0.2 mg
Host name: Mouse
Clone: NI 194-3 (A89-040)
Isotype: IgG1
Immunogen:

Description

Description: The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100; in Western blotting from 1:200 upwards.

Additional Text

Antibody Clonality

Monoclonal

Application Notes

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Storage Note

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4C, not refrozen, and preferably used the same day