Mouse anti Human IgE (class specific), conjugated with FITC

Catalogue Number: MAHU/IGE/FITC-NMB

Manufacturer:Nordic MuBio
Type:Monoclonal Secondary Antibody - Conjugated
Shipping Condition:RT
Unit(s): 0.2 mg
Host name: Mouse
Clone: NI 307
Isotype: IgG1
Immunogen:

Description

Description: The reactivity of the antiserum is restricted to a isotype specific determinant of the IgE molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunofluorescence staining of cytoplasmic Ig. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. These data should be interpreted as general recommendations only.

Additional Text

Antibody Clonality

Monoclonal

Application Notes

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Storage Note

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4C, not refrozen, and preferably used the same day.