Mouse anti Human IgG1 (Fc subclass specific), conjugated with Biotin
Catalogue Number: MAHU/IGG1/BIO-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.2 mg |
| Host name: | Mouse |
| Clone: | NI 132 (HP 6186) |
| Isotype: | IgG1 |
| Immunogen: |
Description
Description: The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40; in ELISA from 1:100 to 1:2000; in Western blotting from 1:200 to 1:4000.
Additional Text
Antibody Clonality
Monoclonal
Application Notes
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
Storage Note
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4C, not refrozen, and preferably used the same day.
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