Mouse anti Human IgG (class specific), conjugated with TRITC
Catalogue Number: MAHU/IGGC/TRITC-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.2 mg |
| Host name: | Mouse |
| Clone: | NI 335 and NI 343 |
| Isotype: | IgG1 |
| Immunogen: |
Description
Description: The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards.
Additional Text
Antibody Clonality
Monoclonal
Application Notes
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Storage Note
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4C, not refrozen, and preferably used the same day.
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