NZYMutagenesis kit

Catalogue Number: MB01201-NZY

Manufacturer:	NZYTech
Type:	Mutagenesis Kits
Shipping Condition:	Dry Ice
Storage Condition:	-70°C
Unit(s):	10 mutations
Application:	PCR, Cln

Overview

Description

Description: NZYMutagenesis kit is designed to make point mutations and delete or insert single or multiple nucleotides in a DNA sequence. The system requires the provision of two synthetic oligonucleotide primers containing the desired mutation. Incorporation of the oligonucleotide primers with NZYProof DNA polymerase generates a mutated plasmid containing staggered nicks, which resists Dpn Idigestion (as the synthetic DNA is not methylated). The resulting mutated plasmid is recovered through transformation of NZYStar competent cells. For a schematic presentation of the mutagenesis protocol, see Figure 1. DNA isolated from dam- Escherichia coli strains, including JM101 and SCS110, is not a suitable template forthe mutagenesis reaction.

Additional Text

Short Description

NZYMutagenesis kit provides a simple and highly efficient method to generate point mutations and delete or insert single (or multiple) nucleotides in any type of plasmid DNA using PCR.

Price

£66.00

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