Alkaline Phosphatase (E. coli)
Catalogue Number: MB01801-NZY
| Manufacturer: | NZYTech |
| Type: | Alkaline Phosphatases |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -70°C |
| Unit(s): | 200 U |
Description
Description: Alkaline Phosphatase (E. coli) non-specifically catalyses the removal of most phosphomonoester bonds from the 5' and 3' termini of DNA and RNA without degrading diphosphate or triphosphate linkages. The enzyme is suitable for removal of terminal monoesterified phosphates from deoxyribo-oligonucleotides. The enzyme is generally used for the removal of single phosphate groups from 5'-ends of linear vectors to prevent re-circularization during cloning or to dephosphorylate DNA prior to kinase labelling protocols. Applications: - Dephosphorylation of DNA - Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis
Additional Text
Short Description
Alkaline Phosphatase is suitable for removal of terminal monoesterified phosphates from deoxyribo-oligonucleotides.
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