DpnI

Catalogue Number: MB07802-NZY

Manufacturer:NZYTech
Type:Restriction Enzymes
Shipping Condition:Dry Ice
Unit(s): 1000 U

Description

Description: Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. DpnI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnI gene from Diplococcus pneumoniae. DpnI activity is blocked by overlapping CpG methylation. The optimum reaction temperature is at 37 °C. Concentration: 10000 U/mL Reaction Buffer: NZYTech provides DpnI with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided). Activity in NZYTech Buffers: NZYBuffer A: 70% NZYBuffer B: 50% NZYBuffer C: 100% NZYBuffer U: 100% Minimize exposure of enzyme to temperatures higher than -20 °C. Neoschizomers: BfuCI, Bsp143I, BstENII, BstKTI, BstMBI, DpnII, Kzo9I, NdeII

Additional Text

Purification

Purified

Short Description

DpnI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnI gene from Diplococcus pneumoniae. DpnI activity is blocked by overlapping CpG methylation. NZYTech's DpnI is an enzyme that acts at 37 °C.