AscI

Catalogue Number: MB23102-NZY

Manufacturer:NZYTech
Type:Restriction Enzymes
Shipping Condition:Dry Ice
Unit(s): 2500 U

Description

Description: Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. AscI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the AscI gene from an Arthrobacter species. AscI activity is blocked by CpG methylation. The optimum reaction temperature is at 37 °C. Concentration: 10000 U/mL Reaction Buffer: NZYTech provides AscI with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided). Activity in NZYTech Buffers: NZYBuffer A: 80% NZYBuffer B: 100% NZYBuffer C: 100% NZYBuffer U: 100% Minimize exposure of enzyme to temperatures higher than -20 °C.

Additional Text

Purification

Purified

Short Description

AscI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the AscI gene from an Arthrobacter species. AscI activity is blocked by CpG methylation. NZYTech's AscI is an enzyme that acts at 37 °C.