TBE (Tris-Borate-EDTA) Buffer 10x, pH 8.3 (liquid)

Catalogue Number: MB27701-NZY

Type:Biological Buffers
Shipping Condition:RT
Storage Condition:RT
Unit(s): 1000 ml
Application: PCR, Elctrphs


Description: Specifications: -Tris 0.9 M 108 g/L -Boric acid 0.9 M 55 g/L -Na2EDTA 0.02 M 43.5 g/L Directions for use: Dilute 100 mL of TBE Buffer, 10x stock solution into 900 mL deionised water to make 1 litre of TBE Buffer. Final concentration is 89 mM Tris base, 89 mM Borate and 2 mM Na2EDTA. On dilution to 1X the user should check pH and adjust as required.

Additional Text

Short Description

In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE (Tris-Borate-Acetate) buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments.