Multiplex PCR NZYTaq 2x Colourless Master Mix

Catalogue Number: MB33601-NZY

Manufacturer:	NZYTech
Type:	PCR Mixes
Shipping Condition:	Blue Ice
Storage Condition:	-70°C
Unit(s):	2 x 1.25 ml
Application:	PCR

Overview

Description

Description: Multiplex PCR NZYTaq 2x Colourless Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube. The Master Mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Amplification efficiency is further improved by the inclusion of a recombinant modified enzyme derived from Taq DNA polymerase, which was engineered to display a hot-start-like PCR capacity and high processivity (fast polymerisation). The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification. Extension time: 30 sec/kb at 72 °C 3' to 5' activity: no Product overhang: 3'-A

Additional Text

Short Description

Multiplex PCR NZYTaq 2x Colourless Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube.

Price

£91.00

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