Taq DNA Ligase
Catalogue Number: MB42601-NZY
| Manufacturer: | NZYTech |
| Type: | Ligases |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -70°C |
| Unit(s): | 2000 U |
Description
Description: Taq DNA Ligase is a NAD+ dependent thermostable DNA ligase that catalyses the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini of two adjacent nucleotides in the context of a double-stranded DNA molecule. The ligation will occur only if the adjacent nucleotides are perfectly paired and have no gaps between them, thus allowing the use of this enzyme as a tool to detect single-base substitutions. As expected from its origin from a thermophilic host, Taq DNA Ligase is active at elevated temperatures (45-65°C). Due to critical differences in substrate specificity, Taq DNA Ligase is not a substitute for T4 DNA Ligase and it is not suitable for cloning applications or adapter ligation/NGS library prep. Taq DNA Ligase is primarily used in molecular diagnostic applications and for direct cloning applications. Applications: - Direct cloning - Ligation-based molecular diagnostic techniques (e.g. Ligase Chain Reaction, LCR; Ligase Detection Reaction, LDR) - Detection of single-base substitutions
Additional Text
Short Description
Taq DNA Ligase is a NAD+ dependent thermostable DNA ligase that catalyses the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini of two adjacent nucleotides in the context of a double-stranded DNA molecule.
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