d-ROMs Test
Catalogue Number: MC002-DCN
| Manufacturer: | Diacron |
| Type: | Biochemical assay |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ml |
| Range: | 40-1000 Ucarr |
| Sensitivity: | 11 Ucarr |
| Sample type: | Plasma, Serum |
| Sample size: | 5 / 10 ul |
Description
Description: Free radicals of oxygen are either single or grouped atoms, one of which, at least, belongs to the oxygen and it possesses one or more 'unpaired' electrons in its/their external orbital(s). Because of its reactivity, free radicals have the potential to 'attack' any organic molecule, generating a class of compounds that are called Reactive Oxygen Metabolites (ROMs). Despite their fair oxidant power, ROMs are relatively more stable compared to their relative free radicals and, therefore, they can be adequately detected and quantified. In the d-ROMs Test, ROMs (hydroperoxides, primarily) of a biological sample, in presence of iron are able to generate alkoxyl and peroxyl radicals, according to the Fenton's reaction. Such radicals react with a chromogenic mixture and this coloured-derivative is photometrically quantified.
Additional Text
Detection Method
Colorimetric 505nm|Colorimetric 546nm
Short Description
Rapid determination of Reactive Oxigen Metabolites (Free Radical Compounds) in plasma and serum
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