BAP Test
Catalogue Number: MC436-DCN
| Manufacturer: | Diacron |
| Type: | Biochemical assay |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 50 ml |
| Range: | 500-6000 µM |
| Sensitivity: | 150 µm |
| Sample type: | Plasma, Serum |
| Sample size: | 10 ul |
Description
Description: In the blood, the defence against harmful attack of reactive species and particularly, against free radicals, is guaranteed by so-called plasma antioxidant barrier. The antioxidant power of the barrier is valuated by measuring the ability of such barrier to oppose the massive oxidant action of hypochlorous acid (HClO). In the OXY-Adsorbent Test, the sample of plasma to be tested undergoes the massive oxidant action of a know-title HClO solution (oxidant solution, R1 Reagent). Such solution is clearly "in excess" compared to the relative "adsorption" ability of sample antioxidant barrier. After a defined interval, residual HClO undergoes the reaction with an alkyl-substituted aromatic amine (A-NH2, that is solubilized in a chromogenic mixture, the R2 reagent of kit). Such amine is oxidized by HClO residue and concomitantly it is transformed in a pink-coloured derivative ([A-NH2•]+). The entity of the plasma barrier which opposite to oxidation derived from the concentration of HClO "adsorbed". It will be measured by difference between sample absorbance and a reagent blank made by only HClO, and it will be expressed as µmol HClO/ml of sample. Besides the "scavengers" antioxidant, with the OXY-Adsorbent Test we can also measure the so called "SHOCK ADSORBERS" (for example mucopolysaccharides, amino acids, proteins, etc.) all the antioxidants not active from the chemical point of view but able to "plug" the oxidant action of ROS, so representing "sacrificial" elements.
Additional Text
Detection Method
Colorimetric 505nm
Short Description
Rapid determination of Biological Antioxidant Potential
SUPPORT
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