BAP Test
Catalogue Number: MC437-DCN
| Manufacturer: | Diacron |
| Type: | Biochemical assay |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ml |
| Range: | 500-6000 µM |
| Sensitivity: | 150 µm |
| Sample type: | Plasma, Serum |
| Sample size: | 10 ul |
Description
Description: In the blood, the defence against noxious attack of reactive species and particularly, against free radicals, is guaranteed by so-called plasma antioxidant barrier. This barrier includes either exogenous (e.g. ascorbate, tocopherols, carotenoids, bioflavonoids, etc.) or endogenous (e.g. proteins, bilirubin, uric acid, cholesterol, GSH, etc.) compounds. Each above-mentioned compound possesses its own antioxidant power (or capacity), and is able in a typical way to oppose, depending on reduction-oxidation potential, to the "oxidant" action of reactive species. Such "power" is associated to the ability of plasma barrier components to give "reducing equivalents" (i.e. either electrons or hydrogen atoms) to reactive species, thus avoiding the abstraction of hydrogen atoms from biomolecules and, therefore, dangerous radicalic chains. Obviously, any injury to "the plasma barrier to oxidation" can result in oxidative tissue damage and, ultimately, it can lead to oxidative stress related diseases and early ageing. The BAP Test is based on the capacity of a coloured solution of ferric ions (Fe3+, R2 Reagent) complexed to a particular chromogen (R1 Reagent), to decolour when the ferric ions (Fe3+) are reduced to ferrous ions (Fe2+). This reduction is generated by an adequate reducing system, that is antioxidant, as is plasma.
Additional Text
Detection Method
Colorimetric 505nm
Short Description
Rapid determination of Biological Antioxidant Potential
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