Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein (MHC-HM401T)
Catalogue Number: MHC-HM401T-KAT
MHC-HM401T Image
MHC-HM401T Image
MHC-HM401T Image
MHC-HM401T Image
MHC-HM401T Image
Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag with an affinity constant of 8.49pM as determined in SPR assay (Biacore T200).
MHC-HM401T Image
The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer is greater than 95% as determined by SEC-HPLC.
MHC-HM401T Image
Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
MHC-HM401T Image
Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 24 months |
| Molecular Weight: | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Uniprot ID
A0A140T913, P61769
Short Description
Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide.
Storage Note
-20 to -80°C for 24 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human MAGE-A4 (HLA-A*02:01) Tetramer, His Tag at 0.5Oeg/ml (100Oel/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. The affinity constant of 8.49pM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
MHC-HM401T Datasheet
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