Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein (MHC-HM402T)

Catalogue Number: MHC-HM402T-KAT

Manufacturer:	Kactus
Shelf Life:	12 months
Molecular Weight:	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Physical state:	Lyophilized
Type:	Other Recombinant Protein
Alias:	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
Host Cell:	HEK293 cells
Shipping Condition:	RT
Unit(s):	100 ug, 500 ug
Application:	ELISA, WB

Overview
Datasheets

Description

Description: Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.

Additional Text

Gene Name

B2M

Gene ID

567

Protein Tag

HIS-AviTag™

Uniprot ID

A0A140T913, P61769

Storage Note

-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Short Description

Recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide.

Biological Activity

Immobilized Human GP100 (HLA-A*02:01) Tetramer, His Tag at 2Oeg/ml (100Oel/Well) on the plate. Dose response curve for Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 28.3ng/ml determined by ELISA. See testing image for detail.

Endotoxin

Less than 1 EU per µg by the LAL method.

MHC-HM402T Datasheet

MHC-HM402T MSDS

Price

£589.00

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