Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein (MHC-HM405T)
Catalogue Number: MHC-HM405T-KAT
MHC-HM405T Image
MHC-HM405T Image
MHC-HM405T Image
MHC-HM405T Image
MHC-HM405T Image
Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag with an affinity constant of 0.09 nM as determined in SPR assay (Biacore T200).
MHC-HM405T Image
The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer was greater than 95% as determined by SEC-HPLC.
MHC-HM405T Image
Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
MHC-HM405T Image
Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Uniprot ID
A0A140T913, P61769
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Short Description
Recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLMWITQC peptide.
Biological Activity
Immobilized Human NY-ESO-1 (HLA-A*02:01) Tetramer, His Tag at 5Oeg/ml (100Oel/Well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. The affinity constant of 0.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
MHC-HM405T MSDS
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