Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HM407B)
Catalogue Number: MHC-HM407B-KAT
MHC-HM407B Image
MHC-HM407B Image
MHC-HM407B Image
MHC-HM407B Image
MHC-HM407B Image
The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.
MHC-HM407B Image
Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
MHC-HM407B Image
Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP TCR with an affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
MHC-HM407B Image
Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide.
Uniprot ID
A0A140T913, P61769
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Anti-AFP (HLA-A*02:01) Antibody, hFc Tag at 2Oeg/ml (100Oel/Well) on the plate. Dose response curve for Biotinylated Human AFP (HLA-A*02:01) , His Tag with the EC50 of 0.19Oeg/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
MHC-HM407B Datasheet
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