Chimeric HLA-A*02:01 (mα3) &B2M&LMP2 (CLGGLLTMV) Tetramer Protein (MHC-HM413T)
Catalogue Number: MHC-HM413T-KAT
MHC-HM413T Image
MHC-HM413T Image
MHC-HM413T Image
MHC-HM413T Image
The purity of Chimeric HLA-A*02:01 (mα3) &B2M&LMP2 (CLGGLLTMV) Tetramer was greater than 95% as determined by SEC-HPLC.
MHC-HM413T Image
Chimeric HLA-A*02:01 (mα3) &B2M&LMP2 (CLGGLLTMV) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
MHC-HM413T Image
Immobilized Chimeric HLA-A*02:01(mα3)&B2M&LMP2 (CLGGLLTMV) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The immunoproteasome, having been linked to neurodegenerative diseases and hematological cancers, has been shown to play an important role in MHC class I antigen presentation. The development of molecular probes that selectively inhibit the major catalytic subunit, LMP2, of the immunoproteasome,LMP2-rich cancer cells compared to LMP2-deficient cancer cells are more sensitive to growth inhibition by the LMP2-specific inhibitor, implicating an important role of LMP2 in regulating cell growth of malignant tumors that highly express LMP2.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Short Description
Recombinant Chimeric HLA-A*02:01(mα3)&B2M&LMP2 (CLGGLLTMV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and CLGGLLTMV peptide.
Uniprot ID
A0A140T913, P01897, P61769
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer, His Tag at 2Oeg/ml (100Oel/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
MHC-HM413T Datasheet
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