Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM431B)
Catalogue Number: MHC-HM431B-KAT
MHC-HM431B Image
MHC-HM431B Image
MHC-HM431B Image
MHC-HM431B Image
The purity of Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer was greater than 95% as determined by SEC-HPLC.
MHC-HM431B Image
Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
MHC-HM431B Image
Immobilized Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Uniprot ID
A0A140T913, P61769
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Short Description
Recombinant Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide.
Biological Activity
Immobilized Biotinylated Human WT-1 (HLA-A*02:01) at 2Oeg/ml (100Oel/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 63.3ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
MHC-HM431B Datasheet
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