Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein (MHC-HM443B)
Catalogue Number: MHC-HM443B-KAT
MHC-HM443B Image
MHC-HM443B Image
MHC-HM443B Image
MHC-HM443B Image
The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.
MHC-HM443B Image
Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
MHC-HM443B Image
Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 51-58 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | PRAME; OIP-4; OIP4; MAPE |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | WB |
Description
Description: PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide.
Uniprot ID
A0A140T913, P61769
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Endotoxin
Less than 1 EU per µg by the LAL method.
MHC-HM443B Datasheet
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