Cytokeratin 8/18 Anti-Human Monoclonal [Clone C-43 + DC10]

Catalogue Number: MSM7-770-P1-EQB

Manufacturer:Enquire Bioreagents
Shelf Life:2 years
Preservative:BSA and Azide
Physical state:Liquid
Type:Monoclonal Primary Antibody
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: C-43 + DC10
Isotype: IgG
Immunogen: Keratin preparation from a human carcinoma (C-43); PMC-42 human breast carcinoma cells (DC10)

Additional Text

Concentration

200ug/ml

Gene Symbol

KRT8 & KRT18

Hu-Chromosome Location

17q21.2 (CK8); 12q13.13 (CK18)

Additional application information

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Molecular Weight

52.5kDa (CK8); 45kDa (CK18)

Specificity

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.