Mouse anti alphaT- Catenin
Catalogue Number: MUB0311P-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | 892_24D2S |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | FC, ICC, IHC-P, IP, WB, IHC-Fr |
Description
Description: Catenins are proteins that connect cadherin/β-Catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-Catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed Human αT-Catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-Catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-Catenins. This indiCates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-Catenins to which it shows an overall amino acid identity of 57%. In cells transfected with αT-Catenin cDNA, interaction with β-Catenin was demonstRated by co-immunoprecipitation. The 892_24D2S antibody anti αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis.
Additional Text
Gene Name
CTNNA3
Gene ID
29119
Uniprot ID
Q9UI47
Antibody Clonality
Monoclonal
Storage Note
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Application Notes
892_24D2S is suitable for immunocytochemistry on acetone fixed cells, immunohistochemistry on frozen and paraffin embedded tissue sections, immunoprecipitation and immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
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