Rat anti Entactin / NiDogen

Catalogue Number: MUB0501P-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	0.1 mg
Host name:	Rat
Clone:	ELM1
Isotype:	IgG2a
Immunogen:
Application:	ICC, WB, IHC-Fr

Overview

Description

Description: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or niDogen). Entactin is a sulphated glycoprotein of approximately 150 kD.

Additional Text

Gene Name

NID1

Uniprot ID

P10493

Gene ID

18073

Antibody Clonality

Monoclonal

Storage Note

The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Application Notes

ELM1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Price

£359.00

-

Quantity
Unit Size

Find out about delivery