Mouse anti Integrin beta 4 (CD104)
Catalogue Number: MUB0909P-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | 58XB4 |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | ELISA, FC, ICC, IF, IP, WB, IHC-Fr |
Description
Description: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. CD104 is a 205kD type I transmembrane glycoprotein, also known as beta 4 integrin. CD104 assoaciates with CD49f (integrin alpha 6) forming a heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation.
Additional Text
Gene Name
ITGB4
Uniprot ID
P16144
Gene ID
3691
Antibody Clonality
Monoclonal
Storage Note
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Application Notes
58XB4 is useful for Enzyme Linked Immuno Sorbent Assays (ELISA), Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western blotting, immunocytochemistry of paraformaldehyde fixed cells and Immubohistochemistry on frozen sections. For protocols see reference 1. Optimal antibody dilution should be determined by titration.
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