Rat anti Laminin
Catalogue Number: MUB1100P-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.1 mg |
| Host name: | Rat |
| Clone: | A5 |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | ICC, IF, IHC-Fr |
Description
Description: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Laminin anchors cells to the basement membrane through interactions with integrins at the cell surface and collagens in the basement membrane.
Additional Text
Gene Name
LAMC1
Gene ID
3915
Uniprot ID
P11047
Antibody Clonality
Monoclonal
Storage Note
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Application Notes
A5 is suitable for immunocytochemistry, immunofluorescence and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
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