Nucleoprotein Interactor-1, hSRP-1 (NPI-1) (MaxLight 490)
Catalogue Number: N7651-ML490-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 114-E12 |
| Isotype: | IgG1 |
| Immunogen: | GST fusion protein. |
| Application: | IF, IP, WB, FLISA |
Description
Description: MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. NPI-1 (nucleoprotein interactor-1, hSRP-1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal. Both subunits of karyopherin are required for protein docking at the NPC; the alpha subunit binds the substrate and the beta subunit acts as an adaptor. Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin beta and promotes the release of both subunits from the docking site. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. The 60 kD NPI-1 was first identified as the human homolog of the yeast SRP-1 protein, by its interaction with influenza virus nucleoprotein. NPI-1 has also been observed in its interaction with Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA) and STAT-1, both of which are transported into the nucleus.
Additional Text
Specificity
Specific for the NPI-1 (nucleoprotein interactor-1, karyopherin alpha 1, hSRP1) protein. On Western blots, it identifies a band at ~60kD. Reactivity has been confirmed with human HeLa and MDBK cell lysates.
Purification
Protein A purified
Antibody Clonality
Monoclonal
N7651-ML490 Datasheet
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