Biotin-11-dATP
Catalogue Number: NU-1175-BIOX-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 x 10 ul |
Description
Description: Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine. Recommended Biotin-16-dATP/dATP ratio for PCR: 50% Biotin-16-dATP/ 50% dATP Please note: The optimal final concentration of Biotin-11-dATP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Biotin-11-dATP/dATP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase [1] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [2] - Primer Extension with Klenow exo- [3,4] - Reverse Transcription with MMLV Reverse Transcriptase [5]
Short Description
γ-[N-(Biotin-6-amino-hexanoyl)]-7-propargylamino-2'-deoxy-7-deaza-adenosine-5'-triphosphate, Triethylammonium salt
Smile String
N1C(=O)N[C@@H]2[C@H]1[C@H](CCCCC(=O)NCCCCCC(=O)NCC#Cc1cn(c3ncnc(N)c13)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)SC2
Molecular Weight
882.71 g/mol (free acid)
Molecular Formula
C30H45N8O15P3S (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-1175-BIOX-L Datasheet
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