8-Azido-ATP

Catalogue Number: NU-155S-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Ph:7.5 +/-0
Physical state:Solution
Type:Nucleotides
Shipping Condition:Blue Ice
Unit(s): 500 ul
Application: Cnjg

Additional Text

Concentration

10-11 mM

Application Notes

Incorporation into RNA by 3'-End Labeling with yeast Poly(A) Polymerase(yPAP)[1] Identification of protein ATP-binding sites[2] The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice - to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) - to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) - to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins

Short Description

8-Azido-adenosine-5'-triphosphate, Sodium salt

Smile String

Nc1c2c(n(c(n2)N=[N+]=[N-])[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)ncn1

Molecular Weight

548.19 g/mol (free acid)

Molecular Formula

C10H15N8O13P3 (free acid)