pCp-Biotin

Catalogue Number: NU-1706-BIO-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Ph:7.5 +/-0
Physical state:Solution
Type:Labelled Nucleotides
Shipping Condition:Blue Ice
Unit(s): 20 ul

Description

Description: pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

Additional Text

Concentration

1.0-1.1 mM

Application Notes

Incorporation into RNA/miRNA by3'-End Labeling with T4 RNA Ligase

Short Description

Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Biotin, Triethylammonium salt

Smile String

Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2)O

Molecular Weight

728.65 g/mol (free acid)

Molecular Formula

C25H42N6O13P2S1 (free acid)