Aminoallyl-dUTP-ATTO-425
Catalogue Number: NU-803-425-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 ul |
Description
Description: Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-ATTO425/ 80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-ATTO425/ 70-50% dTTP (Nick Translation) Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Short Description
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 425, Triethylammonium salt
Smile String
O=C(OCC)c1cc2c(oc1=O)cc1N(CCCC(=O)NC/C=C/c3cn(c(=O)[nH]c3=O)[C@H]3C[C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)C(C)(C)CC(C)c1c2
Molecular Weight
906.66 g/mol (free acid)
Molecular Formula
C34H45N4O19P3 (free acid)
NU-803-425-S MSDS
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