Aminoallyl-dUTP - Solid
Catalogue Number: NU-803-5-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 mg |
Description
Description: Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasksto introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging
Additional Text
Application Notes
Incorporation into DNA/cDNA byPCR with Taq polymerase[1], in-house dataNick Translation with DNAse I/ DNA Polymerase I[2]Primer Extension with Klenow exo- [3]3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4]Reverse Transcription with MMLV Reverse Transcriptase[2]
Short Description
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, Sodium salt
Smile String
NC/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
CAS Number
116840-18-7
Molecular Weight
523.22 g/mol (free acid)
Molecular Formula
C12H20N3O14P3 (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-803-5 Datasheet
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