Catalogue Number: NU-803-550-L-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Ph: | 7.5 +/-0 |
Physical state: | Solution |
Type: | Labelled Nucleotides |
Shipping Condition: | Blue Ice |
Unit(s): | 5 x 10 ul |
Description: Aminoallyl-dUTP-ATTO550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO550/dTTP ratio Nick Translation: 30-50% Aminoallyl-dUTP-ATTO550/ 50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
1.0-1.1 mM
Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data
[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)([O-])O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN(C(=O)c1c(C2=c3cc4C(CC([NH+]=c4cc3Oc3cc4NC(CC(c4cc23)C)(C)C)(C)C)C)cccc1)C)O
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 550, Triethylammonium salt
1098.97 g/mol (free acid)
C49H61N6O17P3 (free acid)