Aminoallyl-dUTP-ATTO-647N
Catalogue Number: NU-803-647N-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 x 10 ul |
Description
Description: Aminoallyl-dUTP-ATTO647N is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO647N/dTTP ratio for Nick Translation: 30-50% Aminoallyl-dUTP-ATTO647N/ 50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Short Description
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 647N, Triethylammonium salt
Smile String
P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN(C(=O)c1c(C2=c3cc4c5=[N+](CCC4)CCCc5c3C(c3c2cc2c(c3)N(C(CC2C)(C)C)CC)(C)C)cccc1)C
Molecular Weight
1151.08 g/mol (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Molecular Formula
C54H69N6O16P3 (free acid)
NU-803-647N-L Datasheet
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