Aminoallyl-dUTP-ATTO-680
Catalogue Number: NU-803-680-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 x 10 ul |
Description
Description: Aminoallyl-dUTP-ATTO680 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Smile String
[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN1c2c(C(=CC1(C)C)CS(=O)(=O)[O-])cc1c(c2)Oc2c(=N1)cc1c(=[N+](CCC1)CC)c2)O
Short Description
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 680, Triethylammonium salt
Molecular Weight
1030.83 g/mol (free acid)
Molecular Formula
C39H49N6O19P3S (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-803-680-L Datasheet
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