Biotin-11-dUTP, Biotin-X-(5-aminoallyl)-dUTP
Catalogue Number: NU-803-BIOX-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 200 ul |
Description
Description: Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/ 50% dTTP Please note: The optimal final concentration of Biotin-11-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data - Primer Extension with Klenow 3'-5' exo- [5] - 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[6] - Reverse Transcription with MMLV Reverse Transcriptase [7]
Short Description
γ-[N-(Biotin-6-amino-hexanoyl)]-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Smile String
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@H]3C[C@@H]([C@H](O3)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)NC(=O)N2
Molecular Weight
862.67 g/mol (free acid)
Molecular Formula
C28H45N6O17P3S (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-803-BIOX-S Datasheet
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