DEAC-dUTP, Aminoallyl-dUTP - DEAC
Catalogue Number: NU-803-DEAC-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 ul |
Description
Description: DEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/ 70-50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Smile String
N(C/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)C(=O)c1cc2c(oc1=O)cc(cc2)N(CC)CC
Molecular Weight
766.48 g/mol (free acid)
Molecular Formula
C26H33N4O17P3 (free acid)
Short Description
Diethylaminocoumarin-5-dUTP Diethylaminocoumarin-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
NU-803-DEAC MSDS
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