DIG-11-dUTP
Catalogue Number: NU-803-DIGXL-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 5 x 25 ul |
Description
Description: Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation: 35% Digoxigenin-11-dUTP/ 65% dTTP Please note: The optimal final concentration of Digoxigenin-11-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Digoxigenin-11-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3] and in-house data - Primer Extension with Klenow exo- [4] - 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [5] - Reverse Transcription with MMLV Reverse Transcriptase [6] Incorporation into RNA by - 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [7]
Smile String
C1[C@@H]2[C@](CC[C@@H]1OCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@@H]3O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@@H](O)C3)c1)([C@@H]1[C@@H](CC2)[C@@]2([C@]([C@@H](C1)O)([C@H](CC2)C1=CC(=O)OC1)C)O)C
Molecular Formula
C43H65N4O21P3 (free acid)
Molecular Weight
1066.91 g/mol (free acid)
Short Description
Digoxigenin-X-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt alkali-stable
NU-803-DIGXL MSDS
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