Rhodamine-12-dUTP, Rhodamine-X-(5-aminoallyl)-dUTP, Rho-green-X-dUTP, RhG-dUTP, R110-dUTP
Catalogue Number: NU-803-RHOX-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 10 ul |
Description
Description: Rhodamine-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Rhodamine-12-dUTP/dTTP ratio for Nick Translation: 30-50% Rhodamine-12-dUTP/ 70-50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Short Description
5/6-Rhodamine-X-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Smile String
[nH]1c(=O)c(cn(c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)/C=C/CNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2ccc(cc2oc2c1ccc(=[NH2+])c2)N)C(=O)[O-]
Molecular Weight
992.71 g/mol (free acid)
Molecular Formula
C39H43N6O19P3 (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-803-RHOX-S Datasheet
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