Catalogue Number: NU-803-TXR-L-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Ph: | 7.5 +/-0 |
Physical state: | Solution |
Type: | Labelled Nucleotides |
Shipping Condition: | Blue Ice |
Unit(s): | 5 x 10 ul |
Description: Aminoallyl-dUTP-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Texas Red/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-Texas Red/ 80-70% dTTP (PCR), 50% Aminoallyl-dUTP-Texas Red/ 50% dTTP (Nick Translation) Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
1.0-1.1 mM
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
TexasRed-12-dUTP 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Texas Red, Triethylammonium salt
P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNS(=O)(=O)c1cc(c(cc1)C1=c2c(Oc3c1cc1c4c3CCCN4CCC1)c1c3=[N+](CCCc3c2)CCC1)S(=O)(=O)[O-].P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNS(=O)(=O)c1cc(ccc1C1=c2c(Oc3c1cc1c4c3CCCN4CCC1)c1c3=[N+](CCCc3c2)CCC1)S(=O)(=O)[O-]
1225.07 g/mol (free acid)
C49H59N6O21P3S2 (free acid)