Aminoallyl-dUTP-XX-ATTO-532

Catalogue Number: NU-803-XX-532-L-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Ph:	7.5 +/-0
Physical state:	Solution
Type:	Labelled Nucleotides
Shipping Condition:	Blue Ice
Unit(s):	5 x 10 ul

Overview
Datasheets

Description

Description: Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Additional Text

Concentration

1.0-1.1 mM

Application Notes

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Short Description

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 532, Triethylammonium salt

Smile String

C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCN(C(=O)c1c(cccc1)c1c2c(oc3c1cc/c(=[NH+]/CC)/c3S(=O)(=O)[O-])c(c(cc2)NCC)S(=O)(=O)O)C

Molecular Weight

1377.22 g/mol (free acid)

Molecular Formula

C53H71N8O25P3S2 (free acid)

NU-803-XX-532-L MSDS

NU-803-XX-532-L Datasheet

NU-803-XX-532-L Further Information

Price

£428.00

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