Catalogue Number: NU-803-XX-532-S-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Ph: | 7.5 +/-0 |
Physical state: | Solution |
Type: | Labelled Nucleotides |
Shipping Condition: | Blue Ice |
Unit(s): | 10 ul |
Description: Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
1.0-1.1 mM
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 532, Triethylammonium salt
C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCN(C(=O)c1c(cccc1)c1c2c(oc3c1cc/c(=[NH+]/CC)/c3S(=O)(=O)[O-])c(c(cc2)NCC)S(=O)(=O)O)C
1377.22 g/mol (free acid)
C53H71N8O25P3S2 (free acid)