5-Propargylamino-dCTP-ATTO-550
Catalogue Number: NU-809-550-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 5 x 10 ul |
Description
Description: 5-Propargylamino-dCTP-ATTO-550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-550/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-550/ 70-50% dCTP Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Smile String
[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)([O-])O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCN(C(=O)c1c(C2=c3cc4C(CC([NH+]=c4cc3Oc3cc4NC(CC(c4cc23)C)(C)C)(C)C)C)cccc1)C)N)O
Short Description
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with ATTO 550, Triethylammonium salt
Molecular Weight
1095.97 g/mol (free acid)
Molecular Formula
C49H60N7O16P3 (free acid)
NU-809-550-L MSDS
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