Catalogue Number: NU-809-BIOX-L-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Ph: | 7.5 +/-0 |
Physical state: | Solution |
Type: | Labelled Nucleotides |
Shipping Condition: | Blue Ice |
Unit(s): | 5 x 200 ul |
Description: Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
1.0-1.1 mM
FISH[1, 2] SNP-analysis[1] Nick-translation[2] TUNEL[3]
γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
N(CC#Cc1c(nc(=O)n([C@H]2C[C@@H]([C@H](O2)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)N)C(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1
C28H44N7O16P3S (free acid)
136632-30-9
859.67 g/mol (free acid)