Biotin-11-dCTP, Biotin-X-5-Propargylamino-dCTP
Catalogue Number: NU-809-BIOX-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 200 ul |
| Application: | TUNEL, FISH, SNP, NckTrans |
Description
Description: Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
FISH[1, 2] SNP-analysis[1] Nick-translation[2] TUNEL[3]
Short Description
γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
Smile String
N(CC#Cc1c(nc(=O)n([C@H]2C[C@@H]([C@H](O2)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)N)C(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1
Molecular Formula
C28H44N7O16P3S (free acid)
CAS Number
136632-30-9
Molecular Weight
859.67 g/mol (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-809-BIOX-S Datasheet
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