5-Propargylamino-dCTP-Cy3

Catalogue Number: NU-809-CY3-L-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Ph:	7.5 +/-0
Physical state:	Solution
Type:	Labelled Nucleotides
Shipping Condition:	Blue Ice
Unit(s):	5 x 10 ul

Overview
Datasheets

Description

Description: 5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/ 70-50% dCTP Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.

Additional Text

Concentration

1.0-1.1 mM

Application Notes

Incorporation into DNA/cDNA by - Primer Extension with Klenow fragment[1] - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Short Description

5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Cy3, Triethylammonium salt

Smile String

N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@H](C1)O)C(=O)CCCCCN1c2ccc(S(=O)(=O)[O-])cc2C(C)(C)/C/1=CC=CC1=*(c2ccc(cc2C1(C)C)S(=O)(=O)O)CC

Molecular Weight

1132.97 g/mol (free acid)

Molecular Formula

C43H55N6O20P3S2 (free acid)

NU-809-CY3-L MSDS

NU-809-CY3-L Datasheet

NU-809-CY3-L Further Information

Price

£361.00

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