Catalogue Number: NU-809-CY5-S-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Ph: | 7.5 +/-0 |
Physical state: | Solution |
Type: | Labelled Nucleotides |
Shipping Condition: | Blue Ice |
Unit(s): | 10 ul |
Description: 5-Propargylamino-dCTP-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy5/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy5/ 70-50% dCTP Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.
1.0-1.1 mM
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Cy5, Triethylammonium salt
N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@H](C1)O)C(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=CC=CC=CC1=*(c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC
1159.01 g/mol (free acid)
C45H57N6O20P3S2 (free acid)