Fluorescein-12-dCTP, Fluorescein-X-5-Propargylamino-dCTP
Catalogue Number: NU-809-FAMX-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 5 x 10 ul |
Description
Description: Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/ 70-50% dCTP Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Short Description
5/6-Fluorescein-X-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
Smile String
[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)C(=O)O)N)O.[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)C(=O)O)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)N)O
Molecular Weight
991.68 g/mol
Molecular Formula
C39H40N5O20P3
NU-809-FAMX-L MSDS
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